Microbial populations may attach to surfaces and form biofilms, which are often covered in an extracellular polymeric matrix of exopolysaccharide (EPS). These are commonly related with pathogen infection, environmental pollution in human health, and industrial processes such as artificial implants and biomedical equipment, petroleum pipelines, textiles, and contact lenses, and pose serious clinical, environmental, and economic consequences. Biofilm architecture confers antibiotic resistance on microorganisms, necessitating the quest for alternative medicines that might impede microbial colonisation. Mechanical force, acid- or alkaline-based detergents, or chemical disinfectants are commonly used to remove and eradicate biofilms from surfaces. However, parameters such as pH, temperature, solubility, concentration, and exposure period have a significant impact on the efficiency of these chemical compounds. Silver nanoparticles (AgNPs) are a potential material for medicinal usage because of their remarkable antibacterial action and hence could be used for its antibiofilm activity. Furthermore, the antibacterial activity of AgNPs may be enhanced when coupled with other materials, particularly graphene oxide, and this combination produces a greater antibacterial effect than pure AgNPs. In this work we employ biobased in situ reduction to generate a reduced graphene oxide (rGO)-based composite containing silver nanoparticles (rGO-AgNPs). Scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive x-ray spectroscopy (EDX), and powder x-ray diffraction were used to characterise the produced rGO-AgNPs (PXRD). Silver nanoparticles supported on GO sheets had a spherical-like morphology and an average size of 6 nm, whereas AgNPs generated without GO had a varied shape and an average size of 60 nm. Because Candida albicans, a prominent opportunistic fungal pathogen, is usually found in biofilms alongside Streptococcus mutans, a mixed culture biofilm was generated and investigated. In mixed culture biofilm models, sub-inhibitory quantities ofthe rGO-AgNPs led to decrease of biofilm development. These findings support demonstrate that green rGO-AgNPs nanocomposites might be used as an antimicrobial coating material to prevent the formation of biofilms in food packaging, medical equipment, or water pipes.